Activity related to artificial pollination pollen quality test methods

Now many of the techniques are the use of artificial pollination pollination method, the following is related to the activity of artificial pollination pollen quality inspection methods.
     1, the required materials, equipment
     100 mesh microscopes, petri dishes, slides, 100ml beaker, glass rod, small medicine spoon, furnaces, incubators, absorbent paper, cotton sticks. Agar, sugar, distilled water, electronic scales, small thin sticks
     2, previously prepared work
     (1), first measuring cup, a glass rod, small medicine spoon, slides, Petri dishes and other glassware dry wash with water.
     (2), were weighed by an electronic balance 1g agar, 15g sucrose.
     (3) Measure the amount of glass distilled water 100ml.
     (4), the bottom of the dish covered with two layers of absorbent paper, absorbing enough water, put two small sticks.
      3, the proportion of the preparation of the medium: agar: Sucrose: distilled water = 1: 15: 100
     Methods: 1g weighed agar containing 100ml of distilled water was poured into the beaker, stirring into the upper edge of the furnace was heated to boiling to a transparent liquid, heating was stopped, into the sucrose, stirring was continued until the sucrose was completely dissolved. With a glass rod dipped in moderation, coated onto a glass slide to 2mm thick appropriate, placed on a small wooden dish, cover the lid aside.
Note:
    (A) must be in strict accordance with procedures.
    (2) distilled water must be guaranteed 100ml, if reduced, at any time additional.
     4, pollen sowing
     Pollen to be tested with a cotton swab dampened with the right amount, at the top of a glass slide coated with a good medium, bouncing gently with your fingers, so that pollen particles are uniformly dispersed into the surface of the medium, cover the dish cover.
      5, pollen culture
The broadcast good pollen dish placed 27.5 ℃ culture incubator, after 3 hours can be observed.
Note:
     (A) throughout the pollen should avoid direct sunlight.
     (2) should be cultured in a dark environment.
      6, to observe the situation of pollen germination
     The plates were removed from the incubator and remove the head 100 slides into the microscope. Count the number of pollen grains were already germinating pollen count and did not germinate.
      7, pollen germination rate is calculated
     Pollen germination of pollen germination rate = D / (germinated pollen + number of pollen grains did not germinate) * 100%

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